Study on Simultaneous Determination of 9 Components in Eclipta Herba from Different Growing Areas by QAMS Method and It’s Quality Evaluation

OBJECTIVE To establish a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous content determination of 9 components in Ecliptae Herba,and to comprehensively evaluate the quality of Ecliptae Herba from different growing areas.METHODS An Agilent ZORBAX Eclipse Plus C₁₈ column (250 mm×4.6 mm,5 μm) was used in the HPLC assay.Acetonitrile-0.2% phosphoric acid aqueous solution with gradient elution was employed as the mobile phase.The flow rate was 1 mL·min^-1,the in jection volume was 20 μL,the column temperature was maintained at 25℃ and the detection wavelength was 210,349 nm.Wedelolactone was used as the internal reference substance to calculate the relative correction factors of the other eight constituents and calculate their contents accordingly.Moreover,the external standard method was used to quantify the 9 components in Ecliptae Herba.Comparison of the difference between the QAMS and external standard method was made.The grey correlation quality evaluation model of Ecliptae Herba was established by grey correlation analysis.RESULTS The relative correction factors of other components measured with wedelolactone as internal reference had good system adaptability,and there was no significant difference between the results obtained by QAMS and the measured values.The relative correlation degree of Ecliptae Herba from different growing areas ranged from 0.440 to 0.747,indicating that there were differences in the quality of Ecliptae Herba from different growing areas.CONCLUSION The established QAMS method is suitable for the content determination of multi index components of Ecliptae Herba.Based on the grey correlation model,the quality of Ecliptae Herba from different growing areas can be evaluated more objectively.