Screening of Angiogenic Inhibitory Activity of Herbal-derived Compounds Based on Zebrafish Model

OBJECTIVE To screen 10 herbal-derived compounds by using zebrafish high-throughput model. METHODS Selection of transgenic vascular fluorescent Fli-1 zebrafish after fertilization for screening of 10 herbal-derived compounds, and the effects on the body vasculature of zebrafish were observed by applying different drug concentrations to zebrafish. The inhibition effects of the compounds on the angiogenesis of zebrafish were quantified by using the area of subintestinal vessels as the main index and the number of subintestinal vessels sprouting as the secondary index. RESULTS The subintestinal vascular area of zebrafish was significantly reduced in the groups of lignocaine(87.34 μmol·L^-1), senkyunolide A(52.02 μmol·L^-1), berberine hydrochloride(537.91 μmol·L^-1), honokiol(9.39 μmol·L^-1) and oleanolic acid(10.95 μmol·L^-1) compared with the normal control group(P<0.01 or P<0.001). The inhibitory effects of lignocaine, senkyunolide A, berberine hydrochloride, oleanolic acid and honokiol on the area of subintestinal vessels were 12%, 17%, 18%, 15% and 24%, respectively. There was no significantly difference in the number of subintestinal vascular outgrowth in zebrafish between each compound and normal control group. The positive control group(5 μmol·L^-1sorafenib) had a significant reduction in subintestinal vascular area of zebrafish compared with normal control group(P<0.05), and its inhibition effect on neovascularization was 22% and the number of subintestinal vascular outgrowth was significantly reduced compared with normal control group(P<0.05). CONCLUSION Lignocaine, senkyunolide A, berberine hydrochloride, oleanolic acid, and honokiol have inhibitory effects on angiogenesis.