Study on HPLC Fingerprint of Codonopsis Radix Combined with Multiple Chemical Pattern Recognition

OBJECTIVE To detected the content of various chemical components in Codonopsis Radix based on HPLC fingerprints,combined with a variety of chemometric statistical analysis to evaluate the overall quality of Codonopsis Radix from multiple sources and batches.METHODS ACE Neptune-C₁₈ column (250 mm×4.6 mm,5 μm) was used,mobile phase was acetonitrile-0.15% formic acid aqueous solution,volume flow rate was 0.8 mL·min^-1,detection wavelength was 260 nm,column temperature was 20℃,for 41 batches Codonopsis Radix medicinal materials from multiple origins were used to construct HPLC fingerprints and combined with chemometric analysis to evaluate the quality of Codonopsis Radix.At the same time,the characteristic components were screened for content detection and analysis.RESULTS The HPLC fingerprint of Codonopsis Radix was established,23 common peaks were identified,and 7 chromatographic peaks were identified.The similarity of the HPLC fingerprints of 41 batches of Codonopsis Radix was between 0.650 and 0.962.Cluster analysis grouped 41 batches of Codonopsis Radix into 2 categories.The 6 main components in the principal component analysis reflected the information of 23 common peaks respectively.In the discriminant analysis of partial least squares,the 9 component peaks that mainly affect the difference between Codonopsis Radix were further screened according to the variable importance in projection.The methodological investigation of the index components showed that the sample recovery rate of each component was 99.09%-103.68%,and the RSD was 1.21%-3.68%.CONCLUSION There are differences in quality of Codonopsis Radix from different origins and sources.The combination of fingerprint,cluster analysis,principal component analysis and partial least square discriminant analysis can comprehensively evaluate the quality of Codonopsis Radix.The establishment of this method can provide a certain reference for the quality control and product evaluation of Codonopsis Radix.