Simultaneous Determination of Nine Active Components in Tongyou Runzao Pills by HPLC with Wavelength Switching Method

OBJECTIVE To determine the content of naringin, neohesperidin, baicalin, wogonoside, baicalein, wogonin, honokiol, magnolol and chrysophanol in Tongyou Runzao pills by HPLC wavelength switching method. METHODS The column of illite Hypersil OSD2 C₁₈(250 mm×4.6 mm, 5 μm) was used; the mobile phase of acetonitril(A)-0.1% phosphoric acid solution(B) was used for gradient elution; the flow rate was 1.0 mL·min^-1; the column temperature was 30℃; the detection wavelength was 275 nm(naringin, neohesperidin, baicalin, wogonoside, baicalein, wogonin) and 220 nm(honokiol, magnolol, chrysophanol); the injection volume was 10 μL. RESULTS The separation of the nine active constituents was good. The linear range was 0.76-22.94, 0.47-14.21, 1.46-43.66, 0.24-7.11, 0.51-15.36, 0.13-3.93, 0.053-1.58, 0.30-8.95, 0.38-11.38 mg·mL^-1for naringin, neohesperidin, baicalin, wogonoside, baicalein, wogonin, honokiol, magnolol and chrysophanol, respectively. The average recoveries(n=6) were 99.27%, 100.04%, 98.90%, 100.10%, 99.67%, 98.75%, 99.88%, 99.59%, 99.92%, respectively with RSDs of the recoveries were 0.12%, 0.15%, 0.41%, 0.18%, 0.28%, 0.27%, 0.65%, 0.46%, 0.24%, respectively.CONCLUSION The method for the determination of 9 components in Tongyou Runzao pills is accurate, sensitive and has good separation effect. It can better evaluate the quality of the drug and provide a scientific basis for the overall quality control of drug.