Determination of Ammonium Chloride in Xiao'er Zhike Syrup by Ion Chromatography

OBJECTIVE To establish an ion chromatography method for determination of ammonium chloride in Xiao'er Zhike syrup. METHODS The chromatographic separation was performed on an IonPac^TMAS11-HC anion exchange column (2.0 mm×250 mm) and IonPac^TM AG11-HC guard column(2.0 mm×50 mm), using suppressed conductivity detector, the temperature of the detection tank and the column were set at 35℃ and 30℃ respectively, the injection volume was 25 μL, an isocratic eluent of 10 mmol·L^-1 KOH was chosen as mobile phase and the flow rate was 0.5 mL·min^-1. RESULTS A good linear relationship between the peak area and its concentration was observed within the range of 10-400 μg·mL^-1(r=1.000 0, n=6); The average recovery was 100.57%(RSD=0.41%, n=6).CONCLUSION The method is specific and accurate, and can be used for determination of ammonium chloride in Xiao'er Zhike syrup.