Study on Inhibition effect and mechanism of Daphnetin on human Triple Negative breast cancer cells MDA-MB-231

OBJECTIVE To explore the inhibitory effect of daphnetin on proliferation, migration and invasion of human triple negative breast cancer cell MDA-MB-231 and its potential mechanism. METHODS Mda-mb-231 cells were cultured in vitro and randomly divided into control group and daphnetin 10, 20, 40 μg·ml^-1 groups. MTT assay was used to detect the MDA-MB-231 cells proliferation. The clone formation of MDA-MB-231 cells in each group was detected by clone formation experiment. Scratch and Transwell assays were used to observe the migration and invasion of cells. Western blotting was used to detect expression of Vimentin, MMP9, Cyclin D1, and CDK4 proteins. RESULTS Compared with the control group, daphnetin groups(10, 20, 40 μg·ml^-1) significantly inhibited the proliferation of MDA-MB-231 cells(P<0.05 or P<0.01), daphnetin groups(10, 20, 40 μg·ml^-1) could inhibit the colony formation of human breast cancer cells MDA-MB-231(P<0.01), the migration and invasion ability of the cells in the daphnetin group(20, 40 μg·ml^-1) decreased remarkably compared with the control group(P<0.01), the protein expression levels of Vimentin, MMP9, Cyclin D1 and CDK4 in the daphnetin group(20, 40 μg·ml^-1) were significantly reduced(P<0.01). CONCLUSION Daphnetin can inhibit the proliferation, migration and invasion of human breast cancer cell MDA-MB-231, and its mechanism may be related to the down-regulation of the expression of Vimentin, MMP9, Cyclin D1 and CDK4.