Determination of the Contents of Five Active Ingredients in Rhapontici Radix from Different Origins by Quantitative Analysis of Multi-components by Single Marker Method

OBJECTIVE To establish quantitative analysis of multi-components by single-marker(QAMS) for determining contents of five ingredients from Rhapontici Radix.METHODS The HPLC method was established and the mobile phase consisted of acetonitrile (A)-0.02%phosphoric acid(B) as mobile phase in a gradient mode.The flow rate was 1.0 mL·min^-1,column temperature was set at 30℃and detection wavelength was 210 nm.Usingβ-ecdysterone as an internal reference,the relative calibration factors of caffeic acid,liquiritin,rosmarinic acid and oleanolic acid were established respectively,and the contents of the other four components were calculated using the relative calibration factors.RESULTS Caffeic acid,β-ecdysterone,liquiritin,rosmarinic acid and oleanolic acid showed good linear relationships within the ranges of 2.09-104.5,2.62-131.2,1.31-65.4,3.06-152.8,1.33-66.6μg·mL^-1(r ≥ 0.999 5),whose average recoveries were 98.68%,99.21%,96.50%,97.44%,100.76%,with the RSDs of 1.12%,0.95%,1.63%,1.60%,2.03%,respectively.The contents of five active ingredients in 12 batches of Rhapontici Radix from different origins determined by QAMS had no significantly different with external standard method.CONCLUSION The method is accurate,reliable and repeatable,which can be used for the quality control of Rhapontici Radix.