Preliminary Evaluation of the Activities Against Acute Drug-induced Liver Injury in Mice by Compound ALI-57

OBJECTIVE To preliminarily evaluate the activities of the compound ALI-57 against acute drug-induced liver injury induced by acetaminophen. METHODS Forty eight C57/BL6 mice were randomly divided into 6 groups with 8 mice in each group as normal group, model group, positive control group(acetylcysteine 300 mg·kg^-1), and compound ALE-57 low, medium, high dose group(75, 150, 300 mg·kg^-1). They were treated with corresponding drugs for 3 d. One hours after the final treatment, acetylcysteine(350 mg·kg^-1) was administrated intraperitoneally to each group except normal group. Fasting without water and blood was collected from celiac vein 8 h later. Alanine aminotransferase(ALT), aspartate aminotransferase(AST) in serum, malondialdehyde(MDA), superoxide dismutase(SOD), glutathione(GSH) and glutamate dehydrogenase(GDH) in liver tissue were determined, HE staining was used to observe the histopathological changes of liver. RESULTS Compared with normal group, the levels of ALT and AST in serum of model group were significantly increased, while the contents of MDA and GDH in liver tissue were increased and the activities of SOD and GSH were decreased. The results of HE pathological staining showed that liver tissue damage was significantly increased. Compared with model group, ALT and AST levels in serum of compound ALI-57 medium and high dose groups were significantly decreased(P<0.05), the contents of MDA and GDH in liver were significantly decreased(P<0.05), and the activities of SOD and GSH were significantly increased in liver(P<0.05), while there were no significant differences between the low dose group and the model group. CONCLUSION Compound ALI-57 has a significant protective effect on mice with acute liver injury induced by APAP, the mechanism maybe related to reducing oxidative stress and improving antioxidant capacity.