Simultaneous Determination of 8 Phenolic Active Components in Fufang Xianzhuli Ye by GC-MS

OBJECTIVE To establish a GC-MS method for the simultaneous determination of 8 phenolic active components such as phenol, guaiacol, 2-methoxy-4-methylphenol, 4-ethyl-2-methoxy-phenol, 4-hydroxy-3-methoxy-styrene, 2,6-dimethoxyphenol, vanillin and syringaldehyde in Fufang Xianzhuli Ye. METHODS The samples were extracted with ether and separated by a DB-35MS UI column(30 m×0.25 mm, 0.25 μm), then the extract was detected by MS under SIM mode. And external standard method was used for quantification. RESULTS Under the optimized experimental conditions, 8 phenolic components were well separated. The linearity was good within the mass concentration range of 0.1-129.4 μg·mL^-1, correlation coefficients were >0.999 0. The detection limits of phenolic active components were <0.043 μg·mL^-1. The spiked recoveries were in the range of 93.76%-101.33% with RSD ≤ 3.08%. CONCLUSION The method is simple, sensitive, accurate and reliable, and can provide some scientific basis for quality control of Fufang Xianzhuli Ye．