Fingerprint and Process Quality Assessment of Raw Herbs, Standard Decoction, Intermediates and Dispensing Granules of Lonicerae Japonicae Flos

OBJECTIVE To establish the HPLC fingerprint and multi-index content determination methods of the raw herbs, standard decoction, intermediates and dispensing granules of Lonicerae Japonicae Flos, and evaluate the correlations among them. METHODS The Agilent Eclipse XDB C₁₈ column(250 mm×4.6 mm, 5 μm) was adopted with acetonitrile-0.1% acetic acid solution as the mobile phase for conducting the gradient elution. The detection wavelength was set at 266 nm with a flow rate of 1.0 mL·min^-1. The injection volume was 10 μL. RESULTS HPLC characteristic spectrum was established for multi-component of raw herbs, standard decoction, intermediates and dispensing granules of Lonicerae Japonicae Flos. There were 15 common characteristic peaks in all fingerprint spectrum of 10 batches of raw herbs, standard decoction, intermediates and dispensing granules of Lonicerae Japonicae Flos, presenting a good correlation, 13 chemical compounds were identified including crypto chlorogenic acid, chlorogenic acid, neochlorogenic acid, secologanic acid, caffeic acid, loganin, secoxyloganin, rutin, hyperoside, cynaroside, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C, and external standard method was used to determine its content.CONCLUSION Correlation is good between fingerprint of raw herbs, standard decoction, intermediates and dispensing granules of Lonicerae Japonicae Flos. The method of simultaneous determination of 13 characteristic components is accurately and reliably. The established quality evaluation model can reflect the overall appearance of four forms of Lonicerae Japonicae Flos, provide data support for quality control of dispensing granules of Lonicerae Japonicae Flos.