Mechanisms of Ganlu Xiaodu Elixir on the Treatment of Mycoplasmal Pneumoniae Pneumonia Based on Network Pharmacology and Molecular Docking

OBJECTIVE To predict the molecular mechanism of Ganlu Xiaodu elixir in the treatment of mycoplasma pneumoniae pneumonia(MPP) by the method of network pharmacology and molecular docking. METHODS The active ingredients and targets of Ganlu Xiaodu elixir were screened by TCMSP and Batmann-TCM and the key targets were obtained by intersection with the disease targets of MPP obtained through GeneCards database and CTD. The protein interaction network was constructed by using STRING and Cytoscape. R software was used for GO analysis and enrichment analysis of KEGG related pathways. The molecular docking verification of the component targets was carried out by Mestro. RESULTS One hundred and forty two active ingredients of Ganlu Xiaodu elixir were screened out, and 143 potential therapeutic targets were obtained. Among them, there were 10 core targets and 20 significantly enriched signaling pathways. Molecular docking results showed that quercetin, β-sitosterol, luteolin and other 10 compounds had high affinity with the core target, community acquired respiratory distress syndrome toxin produced by mycoplasma pneumoniae and cellular receptor Annexin A2. CONCLUSION This study preliminarily revealed the potential targets, biological processes and signal pathways involved in the treatment of MPP by Ganlu Xiaodu elixir, suggesting that it has the characteristics of multiple components, multiple targets and multiple pathways, laying a foundation for further research.