Simultaneous Determination of 11 Components in Yinhua Mangguo Tablets by HPLC-MS/MS

OBJECTIVE To establish a method for the simultaneous determination of chlorogenic acid, macranthoidin B, dipsacoside B, mangiferin, quercetin, caffeic acid, luteolin-7-O-glucoside, ferulic acid, platycodin D, hesperidin and liquiritinin in Yinhua Mangguo tablets by HPLC-MS/MS. METHODS The separation was performed on an Agilent Porshell SB-C₁₈ column(4.6 mm×50 mm, 2.7 μm). The mobile phase was consisited of acetonitrile-0.1% formic acid by gradient elution at 30 ℃ column temperature. The flow rate was 0.5 mL·min^-1. For quantitation, electrospray ionization source was applied and the mass spectrometer was operated in multiple reaction monitoring modes. RESULTS Eleven components showed good linearity with correlation coefficient >0.999. The average recoveries(n=9) ranged from 98.15% to 98.81% with RSD≤0.6%. The contents in 4 batches of samples were 4.97-5.24, 8.51-9.28, 2.97-3.23, 6.31-6.68, 0.26-0.30, 0.80-0.93, 0.22-0.25, 0.33-0.38, 0.95-1.07, 4.08-4.36, 1.93-2.21 mg·g^-1, respectively. CONCLUSION The method was simple, sensitive, accurate and reliable for the quality control of Yinhua Mangguo tablets.