Identification of Chemical Compounds in Pipa Qingfei Decoction by LC-Q-TOF-MS/MS

OBJECTIVE to identification the chemical compounds in Pipa Qingfei decoction by LC-Q-TOF-MS/MS. METHODS standard freeze-dried powder of Pipa Qingfei decoction was prepared and extracted by 10% ethanol solution. Inertsustain C₁₈ (250 mm×4.6 mm, 5 μm) chromatographic column was applied with acetonitrile(A)-0.3% formic acid aqueous solution(B) as mobile phase for gradient elution, flow rate was 0.4 mL·min^-1, and column temperature was 30 ℃, with electron spray ionization(ESI). RESULTS Through the integration of literature, comparison of reference materials, and database query and inference, 63 compounds, including 16 alkaloids, 8 flavonoids, 2 sesquiterpenoid glycosides, 2 phenolic acids, and 8 other substances, were preliminarily identified from the material baseline of Pipa Qingfei Drink. CONCLUSION This method can be used to identify the chemical components of Pipa Qingfei decoction, clarify the structure and composition of the compound, and provide a reference for subsequent quality control.