Study on Mechanism of Sirt3/Sod2 Signaling Pathway Mediated Bakuchiol Against Aortic Apoptosis in Diabetic Mellitus Rats

OBJECTIVE To study the protective effect and mechanism of bakuchiol in diabetic aortic artery, and clarify the role of Sirt3/Sod2 signaling pathway in this process. METHODS SD rats were randomly divided into control group, diabetic mellitus group, diabetic mellitus and bakuchiol co-treatment group. Endothelial cells were divided into high fat and high sugar group, high fat and high sugar + 3-TYP group, high fat and high sugar + bakuchiol group, and high fat and high sugar + bakuchiol + 3-TYP group. Fasting blood glucose in rats was detected by OGTT. HE staining was used to observe the pathological changes of aortic vascular wall. The expression of Sirt3 in aortic artery was observed by fluorescence staining. Western blotting was used to detect the expression of Sirt3, Ac-Sod2, Cleaved caspase-3, Bax and Bcl-2 proteins in aortic tissue and endothelial cells. Then the mechanism of bakuchiol against aorta apoptosis in diabetes rats was studied by 3-TYP, an inhibitor of Sirt3. RESULTS In diabetes, the aortic wall tissue was disordered, and the number of cells decreased. The expression of Sirt3 and Bcl-2 protein decreased, while the expression of Ac-sod2, Cleared caspase-3 and Bax protein increased. However, bakuchiol could significantly improve the disorder of aortic wall tissue caused by hyperglycemia. The expression of Sirt3 and Bcl-2 protein increased, while the expression of Ac-sod2, Cleared caspase-3 and Bax protein decreased. After the endothelial cells injured by high fat and high glucose, the expression levels of Sirt3 and Bcl-2 proteins were decreased, and the expression levels of Ac-sod2, Cleaved caspase-3 and Bax proteins were increased. However, bakuchiol could significantly increase the expression levels of Sirt3 and Bcl-2 proteins, and decrease the expression levels of Ac-sod2, Cleaved caspase-3 and Bax proteins. The protective effect of bakuchiol was inhibited by 3-TYP. CONCLUSION Bakuchiol co-treatment could significantly inhibit the aortic artery damage caused by diabetic mellitus and reduced cell apoptosis by activating Sirt3/Sod2 pathway.