Study on Chemical Equivalence and Anti-inflammatory Equivalence of Aerial Parts of Tetrastigma Hemsleyanum Dispensing Granules and Decoction Pieces

OBJECTIVE To explore the correlation and equivalence between Tetrastigma hemsleyanum dispensing granules and decoction pieces based on the characteristic spectrum, and evaluate the anti-inflammatory pharmacological equivalence. METHODS The common pattern of the HPLC characteristic spectra of the decoction pieces, decoction, intermediates, and dispensing granules of the aerial parts of Tetrastigma hemsleyanum was established. Based on the common pattern and the content of the main active ingredients, an equivalent-ratio model was built. The migration rates were calculated. The similarity of the common active ingredient groups and the equivalent correlation between the dispensing granules and the decoction pieces were analyzed. A cotton-pellet-induced rat granuloma model was used to conduct a pharmacodynamic study on the anti-inflammatory effect. The pharmacological equivalence between the dispensing granules and the decoction pieces of the aerial parts of Tetrastigma Hemsleyanum was evaluated. RESULTS The common pattern of the HPLC characteristic spectra was established. There were 10 characteristic common peaks. The similarity between the decoction and the dispensing granules was >0.900. The control decoction was prepared from decoction pieces with an average migration rate of 14.97%-23.89%. The dispensing granules were prepared from the decoction pieces, each the average mobility of the components was 54.61%-92.30%. The average equivalent ratio of the three batches of dispensing granules and decoction pieces was 3.72. Taking 80% of the average value as the result, the equivalent ratio between the dispensing granules and the decoction pieces was 2.98. This meant that 1 g dispensing granules was equivalent to 3 g decoction pieces. compared with the model group, the dispensing granules and the decoction of the aerial parts of Tetrastigma hemsleyanum could significantly reduce the weight of rat granuloma(P<0.05). The inhibition rate on the proliferation of inflammatory tissue of cotton ball granuloma in rats was 15.70% and 37.27% in the low and high dose groups of dispensing granules, and 14.59% and 35.73% in the low and high dose groups of decoction. This implied that both the granules and the decoction had anti-inflammatory effect. Their inhibition rates had no significant difference, and were both dose-dependent. This implied that the dispensing granules and the decoction were pharmacological equivalent. CONCLUSION The common medicinal component groups of the aerial parts of Tetrastigma hemsleyanum dispensing granules and decoction pieces, decoction are similar, and the dose-effect correlation between dispensing granules and decoction pieces is 1:3. The dose-effect ratio of the medicine is equivalent to the same medicinal effect correlation.