Simultaneous Determination of Contents of 7 Triterpenoid Acids in Lingzhi Capsule by LC-MS/MS

OBJECTIVE To establish the LC-MS/MS method for simultaneous determination of contents of 7 triterpenoid acids in Lingzhi capsule. METHODS The determination was performed on Agilent Zorbax SB-C₁₈ column(3.0 mm×150 mm,0.35 μm) with column temperature of 40℃. The injection volume was 5 µL. Ion source was electrospray ion source. Negative ion mode monitoring was conducted and chloramphenicol was used as internal standard. MRM mode was adopted. The acetonitrile-water(38:62) was used as mobile phase with the flow rate of 0.3 mL·min^-1. RESULTS The linear ranges of ganoderic acid A, B, C₂, F, G, ganoderenic acid D and lucidenic acid A were 4.972-497.2, 4.927-492.7, 4.949-494.9, 4.978-497.8, 4.990-499.0, 5.070-507.0, 5.089-508.9 ng·mL^-1(r ≥ 0.999 8), respectively. RSDs of instrument precision, stability and reproducibility tests were all<5%. The recoveries rate were 98.4%-101.8% (RSD=1.28%-3.71%, n=6). CONCLUSION The method is simple, rapid and reproducible, and can be used for content determination of 7 triterpenoid acids in Lingzhi capsule. It can be used for quality control of Lingzhi capsule.