Study on Quality Evaluation of Jinhua Qinggan Granules Based on Fingerprint, Pattern Recognition and Multi-components Determination

OBJECTIVE To establish the fingerprint of Jinhua Qinggan granule(JHQGG) by HPLC, analyze them with chemical pattern recognition technology, and determine the contents of chlorogenic acid, mangiferin, forsythoside A, baicalin, arctiin, wogonoside and ammonium glycyrrhizinate in JHQGG, in order to provide a scientific basis for its quality evaluation. METHODS The HPLC fingerprint of JHQGG was performed on Agilent Eclipse XDB-C₁₈(250 mm×4.6 mm, 5 μm) column with acetonitrile-0.1% phosphoric acid solution for gradient elution, the fingerprint data was analyzed by the similarity evaluation system of traditional Chinese medicine chromatographic fingerprints combined with cluster analysis(CA) and principal component analysis(PCA). Furthermore, the contents of seven components in JHQGG were determined. RESULTS The HPLC fingerprint of 13 batches of JHQGG was established. The similarity were all >0.98. Seventeen common peaks were marked and 8 of them were identified, including chlorogenic acid(peak 2), mangiferin(peak 4), glycyrrhizin(peak 6), forsythiaside A(peak 8), baicalin(peak 12), arctiin(peak 13), wogonin(peak 16) and ammonium glycyrrhizinate(peak 17). CA and PCA were used to classify the 13 batches of JHQGG into two groups. The results of multi-component quantitative analysis methodology showed that the linear relationship(r²> 0.999 8), precision(RSD ≤ 1.41%, n=6), repeatability(RSD ≤ 1.31%, n=6), stability(RSD ≤ 2.08%, 24 h) of seven components were good, the average recoveries were between 97.11% and 102.41%, and the RSD were in the range of 0.80%-1.98%. The contents of chlorogenic acid, mangiferin, forsythoside A, baicalin, arctiin, wogonoside and ammonium glycyrrhizinate in 13 batches of JHQGG were in the range of 2.109-3.008, 0.378-0.616, 1.379-3.629, 10.146-18.485, 11.789-19.210, 2.208-3.526, 1.308-1.810 mg·g^-1, respectively. CONCLUSION The method of HPLC fingerprint combined with pattern recognition and multi-components simultaneous determination is rapid, simple and reproducible, which can provide a reference for the quality evaluation of JHQGG.