Study on HPLC-PDA Full-wavelength Maximum Characteristic Chromatogram of Congrong Shujing Granules

OBJECTIVE To explore the HPLC-PDA full-wavelength maximum method and to establish characteristic chromatogram of Congrong Shujing Granules, so as to provide a basis for evaluating its overall quality. METHODS The HPLC-PDA 210-800 nm full-wavelength maximum method was used to detect. Agilent Zorbax Eclipse XDB-C₁₈ column (250 mm×4.6 mm, 5 μm) was used as the stationary phase, and acetonitrile(A)-0.1% phosphoric acid solution(B) was used as the mobile phase. Gradient elution was performed, and PDA maximum value graph showed that the flow rate was 1.0 mL·min^-1, the column temperature was 25℃, the injection volume was 20 μL, and the elution time was 60 min. RESULTS The common mode of the HPLC-PDA full-wavelength maximum characteristic chromatogram of Congrong Shujing Granules with salvianolic acid B as the reference peak was established, and 20 common characteristic peaks were identified and assigned to 6 of them. The analyzed 10 batches of Congrong Shujing Granules had a good similarity with the common pattern, and the similarity was all >0.950. CONCLUSION The first established HPLC-PDA full-wavelength maximum method characteristic chromatogram has good reproducibility and high sensitivity. It can more accurately characterize and extract the information of the medicinal component group of Congrong Shujing Granules, and lay the foundation for formulating the quality standard of Congrong Shujing Granules. It provides a new method that can be used for reference for the establishment of the characteristic chromatogram of Chinese herbal compound preparations with complex systems.