Characteristic Spectra and Multi-component Content Determination of Different Root Forms of Lindera Aggregate

OBJECTIVE To establish a method for the qualitative analysis of specific chromatogram and quantitative analysis of 6 components of different forms of Lindera aggregate, and to provide a reliable method and basis for the quality control of Lindera Aggregate. METHODS The determination was performed on Agilent Zorbax SB-C₁₈(4.6 mm×250 mm, 5 μm) column with mobile phase consisted of acetonitrile-0.2% phosphoric acid(gradient elution) at the flow rate of 0.8 mL·min^-1. The detection wavelength was set at 210 nm. The column temperature was set at 30℃, and the injection volume was 10 μL. The HPLC specific chromatogram were analyzed and the similarity was evaluated with "Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine". The contents of norisoboldine, linderalactone, boldine, isolinderalactone, linderane and kaempferol were determined. RESULTS Compared with the common pattern of fingerprints, the similarities of 22 batches of samples were>0.980 with 20 common peaks. The linear range was 12.7-12 700 μg·mL^-1(r=0.999 1) for norisoboldine, 5.3-1 040 μg·mL^-1(r=0.999 2) for linderalactone, 7.5-6 210 μg·mL^-1(r=0.999 5) for boldine, 10.25-8 200 μg·mL^-1(r=0.999 3) for isolinderalactone, 5.5-2 200 μg·mL^-1(r=0.999 3) for linderane, 6.85-1 370 μg·mL^-1(r=0.999 2) for kaempferol. The contents of norisobordine, linderane and boldine in root tuber of Lindera aggregate were higher than those in taproot of Lindera aggregate, while the contents of isolinderactone were lower in root tuber than taproot, there was no statistically significant difference in the contents of linderalactone and kaempferol. CONCLUSION The established method is accurate, stable and reliable. It can provide reference for a well-acceptable strategy for chemical comparison of taproot of Lindera aggregate.