Analysis on the Monosaccharide Composition of Toddalia Asiatica Lam Polysaccharides and Study on Its Effect on Macrophage Foaming

OBJECTIVE To study the monosaccharide composition of Toddalia asiatica Lam polysaccharides(TALP) and the effect of polysaccharides on the formation of macrophage foam, and to explore the possible mechanism of TALP against atherosclerosis. METHODS The polysaccharides were extracted by water extraction and alcohol precipitation. The polysaccharides were obtained by drying after removing the protein. TALP were purified by Sephadex-G200. TALP were purified by Sephadex-G200 to obtain TALP component 1(TALP1) and component 2(TALP2). The composition and structure of TALP were determined by PMP-HPLC and FT-IR. Human peripheral blood mononuclear cell line(THP-1) was induced to differentiate into macrophages by phorbol tetradecanoate(PMA) and divided into control group, oxidized low density lipoprotein (ox LDL) group and ox LDL+TALP1 group. Then oil red O staining was performed and the staining area of oil red O staining was analyzed. RESULTS The infrared spectra of TALP showed typical absorption peaks of polysaccharides. TALP was a water-soluble heteropolysaccharide composed of glucose, mannose, arabinose, rhamnose, galactose, glucuronic acid, galactose, fructose and two other unknown monosaccharides. The results of oil red O staining showed that there was no oil red O staining in the control group. The staining area of oil red O in the Ox-LDL group increased significantly. After TALP1 intervention, the staining area of oil red O in the Ox-LDL+TALP1 group decreased. CONCLUSION TALP is a heteropolysaccharide containing glucose, mannose, arabinose, rhamnose, galactose, glucuronic acid, galactose, fructose, etc. TALP1 inhibits macrophage foaming.