Study on the Effect of Soybean Milk on the Intestinal Absorption Characteristics of Legumes Traditional Chinese Medicine and Relevant Mechanism

OBJECTIVE To determine the effect of Chinese dietary soybean milk on intestinal absorption of legume traditional Chinese medicine, and to guide the rational diet during clinical medication. METHODS Pharmacokinetics was used to study the effect of soybean milk on the absorption in vivo of the representative flavonoids of Puerariae lobatae radix, Astragali radix, Glycyrrhiza radix et rhizoma and Sophorae flos. In vivo inte stinal perfusion was used to study the effect of soybean milk on the absorption of the effective ingredients and its change rule with time. Eversion intestinal sac method and enzyme inhibitor method were used to study the effect of P-gp and MRP2 protein in this process. UHPLC-QQQ-MS/MS method was used to detect the contents of puerarin, rutin, glycyrrhizin and anthocyanin in plasma and intestinal fluid and the pharmacokinetic parameters, cumulative absorption capacity and absorption rate constant of each component in rats were calculated. RESULTS Soybean milk significantly reduced the AUC_0-t, C_max of the functional components in rats. The simultaneous perfusion of soybean milk and liquid medicine could significantly inhibit the absorption of the four functional components in each intestinal segment. The pre-perfusion of soybean milk for 2 h also significantly inhibited the absorption of puerarin, anthocyanin and glycyrrhizin. The pre-perfusion of 0.5 h and 1 h had no significant effect on the absorption of the functional components. Verapamil and probenecid had no significant effect on the cumulative absorption, absorption rate constant and apparent permeability of the four components. CONCLUSION Soybean milk significantly inhibit the absorption and transport of flavonoids from Puerariae lobatae radix, Astragali radix, Glycyrrhiza radix et rhizoma and Sophorae flos in rats in vivo, which may not be related to P-gp and MRP2. The specific mechanism of action needs further study. When taking drugs containing legumes, it is avoided to taking soybean milk at the same time.