Study on Effect of Zhonghua Dieda Pill on Fracture Healing of Zebrafish

OBJECTIVE To study the antifracture healing, anti-inflammatory, angiogenesis and repair of cartilage damage effects of Zhonghua Dieda pill by using zebrafish. METHODS The fracture model was established by squeezing the tail fin of zebrafish by a blunt edge knife, and the effect of Zhonghua Dieda pill on fracture healing and anti-inflammatory was then evaluated; transgenic vascular fluorescence zebrafish was used to evaluate the angiogenic effect; cartilage damage model was established by treating zebrafish with dexamethasone, and then the effect of cartilage damage reparing was evaluated; the effects of Zhonghua Dieda pill on the gene expressions of TNF-α, IL-1β, BMP-2 and BMP-7 were measured by Q-PCR. RESULTS Compared with the model control group, the fracture healing effect of the Zhonghua Dieda pill was 20.8%(P<0.05) when concentration was 2 mg per tail. With down-regulating the expression of TNF-α and IL-1β genes(P<0.05 or P<0.01), the anti-inflammatory effect of Zhonghua Dieda pill was 44.6%, 50.6% and 52.6% at the 15.6, 31.3 and 62.5 mg·mL^-1 concentration(P<0.05); the angiogenesis effect of Zhonghua Dieda pill was 19.7% and 49.9% at the 1 000, 2 000 mg·L^-1 concentration groups(P<0.01 or P<0.001); Zhonghua Dieda pill showed cartilage damage repairing effect by up-regulating the expression of BMP-2 and BMP-7 genes and result in 42.0% effect of cartilage damage repairing on zebrafish model at 500 mg·mL^-1 concentration(P<0.05). CONCLUSION Zhonghua Dieda pill can promote fracture healing, anti-inflammatory, angiogenesis and cartilage damage repairing in zebrafish, and play an anti-inflammatory role by down-regulating the expression of TNF-α and IL-1β and play a cartilage repairing by up-regulating the expression of BMP-2 and BMP-7 genes.