Analysis of the Role of CKMT1A on Non-small Cell Lung Cancer Chemotherapy Resistant Based on Data Mining

OBJECTIVE To explore the role of creatine kinase mitochondrial 1A(CKMT1A) on non-small cell lung cancer(NSCLC) chemotherapy resistant.METHODS HPA, GEPIA, GEO databases or online tools were used to explore the expression of CKMT1A on NSCLC or cisplatin resistant NSCLC cell line, the difference between two groups was evaluated by unpaired t test. Pathway enrichment analysis of CKMT1A interacted genes was conducted by STRING and DAVID 6.8, enriched P value was evaluated by Fisher Exact Test. miRNA-mRNA interaction analysis was conducted by microRNA.org and Targetscan to further prove the role of CKMT1A on NSCLC chemotherapy resistant. The relation of CKMT1A expression or microRNA expression and NSCLC overall survival(OS) were conducted by Kaplan Meier-plotter using log-rank test. COREMINE was used to analyze the relation of enriched pathways and NSCLC chemotherapy resistant and the relation of microRNA and NSCLC chemotherapy resistant. RESULTS CKMT1A were over expressed in NSCLC and cisplatin resistant NSCLC cell line(P=0.000). High expression of CKMT1A was significantly associated with lower OS in NSCLC(P<0.05). Metabolic pathways, especially arginine and proline metabolism pathway were enriched among genes interacted with CKMT1A(P<0.05), which was closely associated with NSCLC chemotherapy resistant. hsa-miR-103 and hsa-miR-107 were target to CKMT1A, and also associated with NSCLC OS and chemotherapy resistant. CONCLUSION CKMT1A over-expression affect clinical prognosis and chemotherapy resistant in NSCLC, and possibly by arginine and proline metabolism pathway or microRNA post-transcriptional regulation.