Study on HPLC Fingerprint of Dryopteridis Crassirhizomatis Rhizoma Dispensing Granules

OBJECTIVE To establish HPLC fingerprint of Dryopteridis Crassirhizomatis Rhizoma dispensing granules. METHODS A gradient elution method with a flow rate of 1.0 mL·min^-1 and a detection wavelength of 330 nm was developed on a HPLC column using C₁₈ as a filler and acetonitrile-1% acetic acid as a mobile phase. Liquid chromatography-mass spectrometry(LC-MS) was used to obtain the molecular weight and molecular ion fragments of the characteristic peaks, and the characteristic peak structure was identified by combining reference materials and literature comparison methods. RESULTS The fingerprint consisted of 12 common peaks, and 7 common peaks were identified, which were neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 4β-car-boxymeth-yl-(–)-epicatchin, eriocitrin, 3-methylbutyryl-phloroglucinol- 4,6-di-C-glucoside, 1-methyl-3-butyryl-phloroglucinol. CONCLUSION The HPLC fingerprint of Dryopteridis Crassirhizomatis Rhizoma dispensing granules. The method displays good stability and repeatability which is suitable for the quality evaluation of Dryopteridis Crassirhizomatis Rhizoma dispensing granules.