Determination of the Related Substances of Potassium Guaiacolsulfonate by HPLC

OBJECTIVE To establish an HPLC method for determination of the related substances of potassium guaiacolsulfonate. METHODS The analysis was performed on an InertSustain C₁₈ column(4.6 mmx250 mm, 5 mm) with the mobile phase consisted of acetonitrile-0.02 mol·L^-1 phosphate buffer(20:80). The flow rate was 1 mL·min^-1. The column temperature kept at 35℃, and the detection wavelength was 279 nm. RESULTS The separation between the two isomers of potassium guaiacolsulfonate could be effectively separated, and the separation between the main component and impurities was good. The calibration curves of potassium guaiacolsulfonate and known impurities were linear in the self-concentration range (r>0.999); and the average recoveries of isomerⅢ, guaiacol and impurity Ⅰwere 101.35%, 100.78%, 99.45%, while RSDs were 0.7%, 0.7%, 1.4%, respectively. CONCLUSION The method is rapid, accurate, sensitive and reliable for determination of the related substances of potassium guaiacolsulfonate.