Simultaneous Determination of Eight Components in Qisang Yigan Pills by HPLC-QAMS

OBJECTIVE To establish a HPLC-QAMS method for the determination of polydatin, resveratrol, emodin, swertiamarin, gentiopicrin, sweroside, calycosin 7-O-β-D-glucopyranoside and formononetin in Qisang Yigan pills. METHODS The separation and detection procedure was performed on an Agilent HC-C₁₈(250 mm×4.6 mm, 5 μm) at 30℃. The mobile phase was composed of acetonitrile-0.1% formic acid aqueous solution using gradient elution at a flow rate of 1.0 mL·min^-1. The detecting wavelength was set at 290 nm for polydatin, resveratrol and emodin, 243 nm for swertiamarin, gentiopicrin and sweroside, and 254 nm for calycosin 7-O-β-D-glucopyranoside and formononetin. Using swertiamarin as an internal standard, the relative correction factors of the other seven constituents were calculated, after which the content determination was made. RESULTS Polydatin, resveratrol, emodin, swertiamarin, gentiopicrin, sweroside, calycosin 7-O-β-D-glucopyranoside and formononetin showed good linear relationships within the ranges of 3.46-69.20, 2.09-41.80, 2.77-55.40, 6.71-134.20, 2.88-57.60, 1.19-23.80, 0.56-11.20, 0.89-17.80 μg·mL^-1(r ≥ 0.999 1), whose average recoveries(RSD) were 100.05%(0.82%), 97.79%(1.07%), 98.41%(0.99%), 99.75%(0.65%), 98.72%(1.21%), 99.07%(1.19%), 96.99%(1.04%), 97.65%(1.38%), respectively. There was no significant difference between the calculated values and the measured values of the external standard method. CONCLUSION The established HPLC-QAMS method is specifical, reliable and can be used to provide a reference standard for quality evaluation of Qisang Yigan pills.