Determination of Morroniside, Loganin, Scutellarin and Naringin in Yishen Gukang Granules by HPLC

OBJECTIVE To determine the content of morroniside, loganin, scutellarin and naringin in Yishen Gukang granules by high performance liquid chromatography(HPLC). METHODS The HPLC method was performed on Kinetex EVO C₁₈column(150 mm×4.6 mm, 5 μm). The gradient elution was adopted with the mobile phase consisting of acetonitrile(A) and 0.5% phosphoric acid aqueous(B), and the injection volume was 10 µL. The detection wavelengths were set at 240 nm (morroniside and loganin), 283 nm(naringin) and 335 nm(scutellarin). RESULTS The calibration curves of morroniside, loganin, scutellarin and naringin were Y=1.897 8×10⁶X‒22 664.29(r=0.999 9), Y=1.792 0×10⁶X+3 040.43(r=0.999 9), Y=3.745 9×10⁶X+328 7(r=0.999 9), Y=1.854 0×10⁶X+654.57(r=0.999 8) and the linear ranges were 0.363 5‒2.544 5, 0.238 2‒1.667 6, 0.087 1‒0.609 4, 0.078 3‒0.548 2 μg, respectively. The average recoveries of morroniside, loganin, scutellarin and naringin were 98.25%, 96.35%, 95.74%, 95.76% and the RSD were 0.85%, 0.92%, 1.06%, 0.67%, respectively. CONCLUSION The method is simple, accurate, stable and reproducible for the quality control of Yishen Gukang granules. The contents of active ingredients in different batches of Yishen Gukang granules are different.