Establishment of Fingerprint and Identification of the Components in Akebiae Fructus Formula Granule by HPLC-Q-TOF-MS

OBJECTIVE To establish the HPLC fingerprint of Akebiae Fructus formula granule and identify common peak components by Q-TOF-MS technique. METHODS Acetonitrile-0.05% formic acid solution was used as a mobile phase to gradient elute Agilent Poroshell 120 EC-C₁₈ chromatographic column(4.6 mm×50 mm, 2.7 μm). The detection wavelength was 210 nm, the column temperature was 30℃, and the flow rate was 0.5 mL·min^-1. At the same time, similarity analysis software was used to analyze the fingerprint. In addition, the characteristic peaks were analyzed and identified by mass spectrometry using Q-TOF-MS anion detection mode with reference substances and literature data. RESULTS The HPLC fingerprint of Akebiae Fructus formula granule has been established. Based on the 4,5-O-Dicaffeoylquinic acid peak as the standard peak, 14 common peaks were identified. Specifically, the similarity range between samples and the similarity range between samples and reference fingerprint were 0.943-0.994 and 0.976-0.988, respectively. Furthermore, high resolution Q-TOF-MS was used to identify common peaks. It was indicated that a total of 8 peak components were identified, including neochlorogenic acid, chlorogenic acid, calceolarioside B, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, Asperosaponin VI and α-Hederin. CONCLUSION This method is sensitive, accurate and reliable, and can be used to predict the quality evaluation of Akebiae Fructus formula granule.