Simultaneous Determination of Ten Components in Shenqi Wuweizi Capsule by HPLC-PAD-ELSD Combined with Chemical Pattern Recognition

OBJECTIVE To develop an HPLC-PDA-ELSD method for the simultaneous determination of calycosin7-O-β- D-Glucopyranoside, ononin, jujuboside A, jujuboside B, astragaloside IV, lobetyolin, spinosin, schisantherin A, schisantherin B, deoxyschizandrin in Shenqi Wuweizi capsule, and to evaluate the quality of Shenqi Wuweizi capsule combined with principal component analysis. METHODS After the sample was extracted with 70% methanol, it was separated on a Waters Symmetry C₁₈ column(4.6 mm×250 mm, 5 μm), and the mobile phase was acetonitrile(A) containing 5% tetrahydrofuran-0.05% formic acid aqueous solution(B) with gradient elution, the flow rate was 1.0 mL·min^-1, and the column temperature was 35℃, the sample volume was 10 μL. Detection wavelength for calycosin7-O-β-D-glucopyranoside, ononin, lobetyolin, spinosin, schisantherin A, schisantherin B, deoxyschizandrin were set at 230 nm and ELSD was used for detection of jujuboside A, jujuboside B and astragaloside IV. The temperature of the drift tube was 60℃. The principal component analysis were conducted for the results of content determination by SIMCA14.1 statistical software. RESULTS Within a certain concentration range, the ten ingredients all showed good linear relationship(r>0.999), and the average recoveries were 98.28%^-102.95% and RSDs were <2.0%. The contents of calycosin7-O-β-D-glucopyranoside, ononin, jujuboside A, jujuboside B, astragaloside IV, lobetyolin, spinosin, schisantherin A, schisantherin B, deoxyschizandrin in 15 samples were 0.136-0.152, 0.296-0.323, 0.334-0.403, 0.074-0.114, 2.401-2.504, 1.455^-1.512, 0.094-0.164, 2.031-2.214, 0.711-0.809, 1.892^-1.973 mg·g^-1, respectively. The samples could be clearly classified into three categories by using PCA. CONCLUSION The established HPLC-PDA-ELSD method for the determination of 10 components in Shenqi Wuweizi capsule is fast, accurate, reproducible and specific, which can provide a reference for the quality control of Shenqi Wuweizi capsule combined with principal component analysis.