Simultaneous Determination of Schisandrin A, Schisandrin B and Deoxyschizandrin in Shengmaiyin Oral Liquid by UPLC

OBJECTIVE To establish quantitative analysis method of effecctive components(schisandrin A, schisandrin B and deoxyschizandrin) in Shengmaiyin oral liquid by UPLC. METHODS The samples were separated on a XBridge^® BEH C₁₈ (2.1 mm×100 mm, 2.5 μm). The mobile phase was composed of acetonitrile(A) and water(B) with gradient elution, the flow rate was 0.5 mL·min^-1 and the injection volume was 5 μL. The column temperature was 40℃ and detection wavelength was 203 nm. RESULTS The linear ranges were 1.950-124.8 µg·mL^-1 for schisandrin A(r=0.999 9), 1.375-88.00 µg·mL^-1 for schisandrin B(r=0.999 9), 1.594-102.0 µg·mL^-1 for deoxyschizandrin(r=0.999 9). The limits of quantify were no more than 0.137 5 µg·mL^-1, limits of detection were no more than 0.068 8 µg·mL^-1; RSDs of instrument precision, stability and reproducibility tests were ≤ 1.18%; the recoveries were 98.65%(RSD=0.82%), 98.47%(RSD=1.21%), 99.24%(RSD=0.99%), respectively. CONCLUSION The method is simple, precise, stable and reproducible, and can be used for simultaneous determination of schisandrin A, schisandrin B and deoxyschizandrin in Shengmaiyin oral liquid.