Study on Protective Mechanism of rhGLP-1(7-36) on Kidney of Rats with Diabetic Kidney Disease

OBJECTIVE To explore the protective mechanism of rhGLP-1(7-36) on the kidney of diabetic kidney disease (DKD) rats. METHODS DKD model rats were randomly divided into model group, rhGLP-1(7-36) low dose group (20 μg·kg^-1), rhGLP-1(7-36) medium dose group(40 μg·kg^-1), rhGLP-1(7-36) high dose group(80 μg·kg^-1), another normal group. Subcutaneous injection was administered for four weeks. Full-automatic biochemical analyzer was used to detect urine microalbumin, serum creatinine, and urinary creatinine levels for 24 h, calculated creatinine clearance. The area under the curve(AUC) was calculated by intraperitoneal glucose tolerance test. HE staining was used to observe the pathological and morphological changes of renal tissue. Western blotting was used to detect the level of SIRT1 protein in kidney tissue. RESULTS Compared with the model group, the creatinine clearance of the rats in the rhGLP-1(7-36) high dose group was significantly improved; the 24 h urine level was significantly reduced(P<0.05); the AUC of intraperitoneal glucose tolerance was significantly reduced(P<0.05); the pathological changes of renal tissue were improved to varying degrees; SIRT1 protein level in renal tissue was significantly increased(P<0.05). CONCLUSION rhGLP-1(7-36) can effectively improve the renal function of DKD rats, and the mechanism may be related to the up-regulation of SIRT1 level in kidney tissue.