Study on Anti-inflammatory Mechanism of Compound Xuanshen Liyan Mixture on Chronic Pharyngitis Model Rabbits Based on ERK/p38 MAPK Signaling Pathway

OBJECTIVE To study the mechanism of Compound Xuanshen Liyan mixture in reducing the inflammatory response of chronic pharyngitis model rabbits. METHODS The chronic pharyngitis model of New Zealand rabbits was established by using the combined factors of 2.5% ammonia injection in pharynx and 0.5 mL turpentine injection in submucosa of pharynx by Peng's modeling method, which were divided into model control group, Jinguoyin group, Compound Xuanshen Liyan mixture group, normal New Zealand rabbits were taken as normal control group. Observed the effect of Compound Xuanshen Liyan mixture on the pathological changes of pharyngeal mucosa in rabbits with chronic pharyngitis, and detected the changes of serum IL-1β, TNF-α and PGE2 by ELISA, and detected the expressions of p38, P-p38, ERK1/2 and P-ERK1/2 in pharyngeal tissues by Western blotting. RESULTS Compared with the normal control group, in the model control group of chronic pharyngitis, the mucosa was not smooth, some cells died and fell off, and the submucosal structure was destroyed, IL-1β, TNF-α and PGE2 in serum were significantly increased(P<0.01), the expression levels of P-p38 and P-ERK1/2 proteins in pharyngeal tissues were significantly increased(P<0.01), indicated a successful modeling. Compared with the model control group, in the Compound Xuanshen Liyan mixture group, the pathological state was improved, the inflammatory indicators IL-1β, TNF-α and PGE2 in serum were all significantly decreased(P<0.01), and the protein expression levels of P-p38 and P-ERK1/2 in pharyngeal tissues were significantly decreased(P<0.01). CONCLUSION Compound Xuanshen Liyan mixture may reduce the inflammatory response of rabbits with chronic pharyngitis by reducing the expression levels of P-p38 and P-ERK1/2 proteins in the MAPK signaling pathway.