Study on Quality Standard of Yao Medicine Combretum Alfredii Hance

OBJECTIVE To establish the quality standard of Yao medicine Combretum alfredii Hance. METHODS The identification of medicinal properties, microscopic characteristics, TLC and content determination method of Yao medicine Combretum alfredii Hance was conducted. The moisture, ash, acid insoluble ash and extract of Combretum alfredii Hance were determined according to Chinese Pharmacopoeia(Volume IV), 2015. RESULTS Combretum alfredii Hance under forest exhibited specific properties in characteristics, microscopic features and TLC results. The Yao medicine Combretum alfredii Hance moisture was 7.38%-12.25%, total ash was 5.81%-10.69%, acid insoluble ash was 0.09%-3.87% and extract was 18.91%-32.29%. Isoorientin had a good linear relationship in 2.750 4-45.8 μg·mL^-1(r=0.999 97), and the average recovery rate was 104.6%. The content of isoorbitin in the 10 batches of medicinal materials ranged from 0.010 5%-0.139 0%. CONCLUSION The established quality standard of Combretum alfredii Hance has good specificity and accuracy. The experimental results can provide a scientific basis for the establishment of the quality standard, promotion and utilization of Yao medicine Combretum alfredii Hance.