Quantitative Analysis of Seven Glycosides in Oxytropis Myriophylla by HPLC

OBJECTIVE To establish the quantitative analysis method for seven glycosides in Oxytropis myriophylla. METHODS Kaempferol-3-O-β-D-glucoside, isorhamnetin-3-O-β-D-glucoside, (3R,4R)-4,7-hydroxy-2',3'-dimethoxyisoflavane-4'-O-β- D-glucoside, 3-hydroxybenzyl alcohol-4-O-β-D-glucoside, 6-methoxycoumarin-7-O-β-D-glucoside, 2-hydroxy-6-methoxyacetophenone -4-O-β-D-glucoside and acetophenone-4-O-β-D-glucoside was taken as evaluation indexes, the quantitative analysis of Oxytropis myriophylla from different habitats was determined by HPLC. The chromatographic column: Topsil C₁₈(250 mm×4.6 mm, 5 μm). The mobile phase was comprised of acetonitrile and water with a gradient elution. The detection wavelength was 261 nm with flow rate at 1.0 mL·min^-1, column temperature at 30 ℃ and injection volume at 20 μL. RESULTS The 7 glycosides all had a good linearity within a certain range(r>0.999 2). The average recoveries were 96.0%, 97.0%, 98.7%, 96.0%, 96.0%, 97.7%, 98.0%, respectively, all of RSDs were<2.0%. Tested compounds and their content change in different samples were 0.192-0.310, 0.215-0.325, 0.504-0.753, 0.128-0.398, 0.151-0.217, 0.354-0.550, 0.132-0.385 mg·g^-1, respectively. CONCLUSION The content detection method established in this experiment can provide reference for comprehensive evaluation of the quality of this medicinal material.