Simultaneous Determination of Five Components in Mongolian Medicine Xanthoceras Sorbifolia Bunge by HPLC Wavelength Conversion Method

OBJECTIVE To establish a method for simultaneous determination of catechin, epicatechin, quercitrin, quercetin, myricetin in Xanthoceras sorbifolia Bunge, and to provide reference and basis for the quality control of Xanthoceras sorbifolia Bunge and its preparation. METHODS HPLC wavelength conversion method was adopted. Agilent Eclipse Plus C₁₈ column(4.6 mm×250 mm, 5 μm)was used; the mobile phase was acetonitrile(A)-0.1% phosphoric acid solution(B) at a flow rate of 1.0 mL·min^-1; the detection wavelength was 0-8 min, 280 nm(catechin, epicatechin), 8-10 min, 257 nm(quercitrin), 10-12 min, 375 nm(myricetin) and 12-18 min, 256 nm(quercetin); the column temperature was 30 ℃; the injection volume was 10 μL. RESULTS The linear ranges of catechin, epicatechin, quercitrin, quercetin, myricetin were 6.64-33.20 μg·mL^-1(r²=0.999 6), 49.04-245.20 μg·mL^-1(r²=0.999 7), 1.28-6.40 μg·mL^-1(r²=0.999 8), 14.4-72.0 μg·mL^-1(r²=0.999 2), 0.84-4.20 μg·mL^-1(r²=0.999 6) respectively, the five components showed good linear correlations; RSD of precision, stability and repeatability were all< 2.0%; the average recoveries were 98.15%(RSD=1.4%, n=6), 102.34%(RSD=1.4%, n=6), 91.90%(RSD=1.3%, n=6), 101.16% (RSD=1.9%, n=6), 94.97%(RSD=1.6%, n=6). CONCLUSION The method is simple and convenient, with high precision, stability and repeatability, and can be used for quality control of Xanthoceras Sorbifolia Bunge.