Study on Quality Standard for Mongolian Medicinal Materials of Ramulus Rubis

OBJECTIVE To establish the quality standard for Mongolian medicine of Ramulus Rubis, and improve its quality control level. METHODS Staking the 8 batches of medicinal materials as the research object, the appearance characteristics were investigated; the microstructure and chemical composition were identified by means of micro-identification and thin-layer chromatography(TLC); the indicator components in medicinal components were analyzed by HPLC; and the general examination items was studied with reference to the appendix of the 2015 edition of People's Republic of China Pharmacopoeia (Part 4). RESULTS The character and microstructure of Ramulus Rubis were described. TLC identification results showed that 8 batches of medicinal materials showing the same color spots were in the corresponding position with reference substance. Caffeic acid, epicatechin and ellagic acid exhibited good linear relationships within the ranges of 0.864-43.20, 7.390-369.5, 7.800-78.00 μg·mL^-1, respectively. RSDs of instrument precision, repeatability, and stability tests were <3%, and the average recoveries were 98.69%, 101.2%, and 103.6%, respectively. According to the experiment results, caffeic acid, epicatechin, ellagic acid should not be <0.025 1, 0.420 6, 0.368 0 mg·g^-1, respectively. Moisture and total ash should not >13.6% and 4.25%, and ethanol extracts should not <2.90%. CONCLUSION This study can provide an experimental basis for the establishment of the quality standard of Ramulus Rubis.