Study on in Vitro Release and Bioadhesion of Breviscapine Temperature-sensitive Nasal in Situ Gel

OBJECTIVE To evaluate the quality of the homemade breviscapine temperature-sensitive nasal in situ gel. METHODS In vitro release of the in situ gel was examined by resonance dialysis, and the bioadhesion of the in situ gel was evaluated by the transport rate of the toad maxillary mucosa cilia and the nasal retention time in rats. RESULTS Breviscapine temperature-sensitive in situ gel met the requirements of nasal administration. In vitro release results showed that the cumulative release of breviscapine temperature-sensitive gel was about 50% at 4 h and the cumulative release was about 90% at 12 h. The investigation of transport rate of the toad maxillary mucosa cilia and breviscapine retention time in rat nasal cavity showed that breviscapine temperature-sensitive gel had bioadhesion. CONCLUSION The breviscapine temperature-sensitive in situ gel releases is complete, it has good bioadhesion, and the retention time in the nose is significantly longer.