Determination of Aluminum in Compound Aminoacid Injection by HPLC-Fluorescence Detector

OBJECTIVE To establish a HPLC method for the determination of aluminum in compound aminoacid injections. METHODS After acid hydrolysis, the free aluminum was combined with 8-hydroxyquinoline in the mobile phase to form a fluorescent complex. HPLC and fluorescent detector was used to determine the content of aluminum by standard curve method. RESULTS The linear relationship of aluminum was good in the range of 5-200 μg·L^-1. The precision and repeatability of RSD were <3%, and the recovery rates were 93.15%-110.07%. CONCLUSION The method is easy to operate, high sensitivity and good accuracy. It provides an effective method for the determination of aluminum in the injection.