Effect of miR-98-5p Targeting CCR7 on the Mobility of Breast Cancer Cell MCF-7 and Its Related Mechanism

OBJECTIVE To investigate the effect and mechanism of miR-98-5p on the mobility of breast cancer cell MCF-7 by targeting CC chemokine receptor 7(CCR7). METHODS MCF-7 cells were divided into control, miR-98-5p mimic, miR-98-5p NC, pc-CCR7, miR-98-5p+pc-CCR7 groups, each group was treated with corresponding miRNA and CCR7 overexpression vector, gene levels of miR-98-5p and CCR7 were measured by RT-PCR, luciferase reporter assay was performed for measuring the relationship between miR-98-5p and CCR7, invasion ability was observed by Transwell, migration was observed by cell scratch. Western blotting was used to determine the protein levels of CCR7, MMP-2, MMP-9, VEGF, E-cadherin, N-cadherin, Vimentin. RESULTS In the Luciferase reporter assay, the luciferase activity of CCR7 WT+miR-98-5p mimic group was significantly reduced compared to CCR7 WT+miR-98-5p NC group. Compared with control group, the level of miR-98-5p, protein level of E-cadherin were increased notably, and the gene and protein levels of CCR7, cell invasion and migration ability, protein levels of MMP-2, MMP-9, VEGF, N-cadherin, Vimentin were decreased significantly in miR-98-5p mimic group, the cell invasion and migration ability, protein levels of CCR7, MMP-2, MMP-9, VEGF, N-cadherin, Vimentin were increased markedly, and the protein level of E-cadherin decreased notably in pc-CCR7 group. Compared with pc-CCR7 group, the cell invasion and migration ability, protein levels of CCR7, MMP-2, MMP-9, VEGF, N-cadherin, Vimentin were decreased markedly, and the protein level of E-cadherin was increased significantly. CONCLUSION miR-98-5p can target CCR7, to inhibit the mobility of breast cancer cell MCF-7, and the mechanism related to epithelial-mesenchymal transition.