Study of Qinba Mushroom Extract (FA-2-b-β) on Apoptosis of Chronic Myeloid Leukemia Primary Bone Marrow Cell

OBJECTIVE To investigate the effect of acidic RNA protein complex (FA-2-b-β) extracted from Qinba mushroom on apoptosis of primary human chronic myeloid leukemia(CML) bone marrow cell line. METHODS The cell proliferation rate was measured by CCK8 method at different time(24, 48, 72 h) points. Flow cytometry was used to detect cell apoptotic rate and cell cycle. Western blotting was used to detect the expression of Bcl-2, Bax, MET, CD44 and β-catenin protein. RESULTS FA-2-b-β inhibited the proliferation of primary CML bone marrow cell line effectively in a dose and time dependent manner. After treated with Qinba mushroom, the apoptosis rates of primary CML bone marrow cell line increased, the expression of Bax increased, and the expression of Bcl-2, MET, CD44, β-catenin decreased. CONCLUSION Qinba mushroom extract inhibits proliferation, induces apoptosis of primary CML bone marrow cell line by regulating the related apoptotic protein. Qinba mushroom has strong anti-leukemia effect in vitro.