Study on Anti-tumor Effect of Loganetin on Pancreatic Cancer Cell BXPC3 and Its Mechanism

OBJECTIVE To investigate the anti-tumor effect of loganetin which is extract from Corni Fructus on pancreatic cancer cell BXPC3 in vitro, and its potential anti-tumor mechanism. METHODS Two groups of experiments were set up, namely the control group and the loganetin group. CCK-8 assay was performed to evaluate change of cell survival and flow cytometry was employed to analyze the effect of loganetin on cell cycle distribution. The migration of human pancreatic cancer cell line BXPC3 was examined by wound healing assay and the invasion was determined using Matrigel-transwell chamber test. Finally, the phosphorylation level of AKT, mTOR and S6 proteins in the AKT-mTOR signaling pathway were detected by Western blotting. RESULTS Compared with the control group, loganetin inhibited the proliferation of human pancreatic cancer cell line BXPC3 in a time and concentration-dependent manner, the inhibition rate of BXPC3 by 100 μmol×L^-1 loganetin for 24 h and 48 h were (21.49±0.01)% and (29.25±0.03)%. Loganetin could block cell cyle in S phase, inhibit migration(P<0.01) and invasion(P<0.01) of cell line BXPC3, and down-regulate the phosphorylation level of AKT, mTOR and S6. CONCLUSION Loganetin may exert its anticancer activity via inhibiting the AKT-mTOR signaling pathway.