Improvement of Content Determination for α-Ketovaine Calcium by HPLC

OBJECTIVE To improve an HPLC method for the content determination of α-ketovaine calcium. METHODS The HPLC method was performed on CAPCELL PAK C₁₈ MGⅡ column(250 mm×4.6 mm, 5 μm) with mobile phase of sodium perchlorate buffer(pH 2.5)-acetonitrile(96:4). Column temperature was 35℃, detective wavelength was 205 nm, and the flow rate was 1.0 mL·min^-1. RESULTS The linear range of α-ketovaine calcium was 0.025-0.185 mg·mL^-1 (r=1.000 0), the average recovery(n=9) was 100.0%. CONCLUSION The method is simple, rapid and accurate with good repeatability, which can be used for the determination of α-ketovaine calcium.