Research on Quality Standards of Testa Arachis

OBJECTIVE To establish quality standards of Testa Arachis. METHODS Character and microscopic identification methods were adopted to observe morphological and histological characters. The moisture, total ash and acid-insoluble ash were determined according to the method of Chinese Pharmacopoeia 2015. TLC was used for qualitative identification. HPLC was used to determine the content of catechin, Sepax BR-C₁₈ column(4.6 mm×250 mm, 5 μm), acetonitrile-water(10:90) as mobile phase, flow rate was 1.0 mL·min^-1. The column temperature was 30℃, and the detection wavelength was 280 nm. RESULTS Testa Arachis had obvious microscopic characteristics and good specificity. TLC with clear spots and good separation. The average contents of water, total ash, acid insoluble ash content, extract and catechin constituent were 8.97%, 6.0%, 0.08%, 23.7% and 0.158%, respectively. In the assay, the catechin was completely separated from the adjacent peaks, and the linear relationship was good. The sample recovery rate(n=6) was 106%, and the RSD was 1.7%. CONCLUSION The established method can be used for the quality control of Testa Arachis.