Research and Application of Dissolved Oxygen Control in Acarbose Fermentation

OBJECTIVE To establish a dissolved oxygen(DO) control strategy and further improve the acarbose titer, investigate the effect of DO concentration changes in various stages of shake flask fermentation on acarbose synthesis of Actinoplanes utahensis. METHODS By adjusting the rotation speed of shaker, reducing the feed concentration and increasing the feed dosage, the DO concentration was regulated and then the effect of DO control strategy on the acarbose titer by Actinoplanes utahensis was determined. RESULTS A DO control strategy was established by simultaneously increasing the rotation speed and reducing the feed concentration in the later fermentation stage. With the established strategy, the maximum acarbose titer reached 8 307 μg·mL^-1 which was 11.98% higher than that of the control group. CONCLUSION Efficient DO regulation can significantly improve acarbose titer of Actinoplanes utahensis. These findings provide support for further improving the acarbose fermentation and reducing the production costs.