Study of 11 Antioxidants in Tobramycin Eye Drops by HPLC

OBJECTIVE To establish an HPLC method for determining the content of 11 antioxidants in tobramycin eye drops, and study the migration of antioxidants from plastic packaging materials to eye drops. METHODS The solid phase extraction column packing with diethenylbenzene polymer was used to concentrate the antioxidants, the HPLC method was set up to determine the contents of antioxidant BHA, XH-245, BHT, 1790, LK-1081, 702, 3114, 1010, 330, 1076 and 168. Putting samples under condition of 50℃ for 0, 5, 10 d to evaluate the antioxidant immigration. RESULTS The 11 antioxidants were well separated. The specificity, the linear range, the precision, the accuracy and the robustness were good. The content of antioxidants were increased after 5, 10 d under 50℃, among which BHA, XH-245, BHT, 3114, 1010 and 1076 had increased significantly. CONCLUSION The method is sensitive, rapid and accurate with good repeatability, which can be used to evaluate the migration of antioxidants from packaging materials and containers to tobramycin eye drops.