Protective Effects of Arctigenin on Rats with Cerebral Ischemia Reperfusion

OBJECTIVE To study the protective effect of arctigenin on rats with cerebral ischemia reperfusion. METHODS Ninety SD rats were randomly divided into 6 groups:blank control group, sham operation group, model group, and arctigenin groups(25, 50 and 100 mg·kg^-1); 15 rats in each group, and different medication intervention were given according to the groups. An improved line-bolt method was used to establish a rat cerebral ischemia-reperfusion model, after the rats' model established successfully, the survival condition and nerve function defect score of the rats were evaluated. After 24 h of cerebral ischemia-reperfusion, two rats were randomly selected and sacrificed for pathological examination of their brain tissue, the ischemic penumbra of the rest rats was stripped off and the levels of SOD, MDA, LDH, IL-1β, IL-6 and TNF-α were detected. RESULTS Compared with the model group, the survival condition of the rats was better and the scores of nerve function defect were significantly lower after intervened with 50, 100 mg·kg^-1 of arctigenin(P<0.01), the number of inflammatory cells around the infarct were significantly decreased, the level of SOD in ischemic penumbra were significantly increased(P<0.01), while the levels of MDA, LDH, IL-1β, IL-6 and TNF-α were significantly decreased. After administration of 25 mg·kg^-1 arctigenin, the number of inflammatory cells around the infarct and the levels of LDH, IL-1β, IL-6, TNF-α were not statistically different from those in the model group, the level of SOD increased significantly(P<0.05), and the levels of MDA was decreased significantly(P<0.05). CONCLUSION Arctigenin can protect cerebral ischemia reperfusion rats through anti-oxidation and anti-inflammatory effects.