Rapid Identification of Chemical Constituents for Buyang Huanwu Decoction and Its Chemical Constituents in Human Plasma and Urine by UHPLC-Q-Exactive-Orbitrap-MS

OBJECTIVE To identify Buyang Huanwu decoction and its chemical constituents in human plasma and urine by UHPLC-Q-Exactive Orbitrap-MS. METHODS The plasma and urine samples of patients were collected after oral administration of Buyang Huanwu decoction. The samples were treated by 80% methanol water. The Hyperil Gold C₁₈ column (1.0 mm×50 mm, 1.9 μm) was used. The mobile phase was water(A)-acetonitrile(B)(containing 0.1% formic acid and 5 mmol·L^-1 ammonium acetate) for gradient elution. The flow rate was 0.3 mL·min^-1, and the column temperature was 40℃. Through the comparative analysis of Buyang Huanwu decoction, human plasma after administration and human urine samples after administration, combined with the mass spectrometry fragmentation law of the compound, the medicinal chemical components in its preparation, medicated plasma and urine were characterized. RESULTS Fifty-five compounds were identified in Buyang Huanwu decoction, 34 compounds in plasma and 23 compounds in urine. These components were flavonoids, benzoic acid, phthalide and saponins. CONCLUSION This study comprehensively elucidate the chemical constituents of Buyang Huanwu decoction and its pharmacochemistry in human plasma and urine, which provides a reference for revealing the real material basis of pharmacodynamics in treating stroke.