Study on Fingerprint of Huoxiang Zhengqi Series Preparations

OBJECTIVE To establish the unified fingerprint of Huoxiang Zhengqi series preparations by HPLC. METHODS The HPLC method was performed on the Waters Symmetry^® C₁₈ column (250 mm×4.6 mm, 5 μm), with acetonitrile and 0.5% glacial acetic acid aqueous solution as mobile phase A and B respectively for gradient elution. The flow rate was 1.0 mL·min^-1, the detection wavelength was 290 nm, the column temperature was set as 40℃. RESULTS The common mode of HPLC fingerprint of Huoxiang Zhengqi series preparations was set up preliminary. There were 18 common peaks in the fingerprints. Six chemical compound as hesperidin, imperatorin, honokiol, isopeulustrin, magnolol and atrctylodin were identified by reference standard attribution method. The similarities of 13 batches Huoxiang Zhengqi series preparations were between 0.802-0.999. CONCLUSION This method has advantages of short time, good reproducibility and high precision, it can be used for the quality control of Huoxiang Zhengqi series preparation.