Protective Effect of Resveratrol on Cognitive Function of Mice Fed Long-term High-calorie Diet and Its Synapse-related Mechanisms

OBJECTIVE To explore the protective effect of resveratrol(RES) on cognitive function of mice fed long-term high-calorie diet(HCD) and its synapse-related mechanisms. METHODS Fifty male C57BL/6 mice were randomly divided equally into control group, HCD group, HCD+25 mg·kg^-1RES group(low dose group), HCD+50 mg·kg^-1 RES group(middle dose group) and HCD+100 mg·kg^-1 RES(high dose group). Six months later, the basic physiological indexes of mice were measured. The cognitive level was measured by Morris water maze and conditional fear test. And the mRNA and protein levels of BDNF, Trk B, SYP and PSD95 in hippocampus were detected by RT-PCR and ELISA, respectively. RESULTS Compared with HCD group, the RES groups demonstrated decreased the body mass, obesity index and fat index of mice, decreased the value of fasting blood glucose and insulin, and HOMA-IR, improved the level of hippocampus-dependent learning and memory, increased the mRNA and protein levels of BDNF, Trk B, SYP and PSD95 in hippocampus, especially for the high dose group. CONCLUSION RES can improve the learning and memory ability of HCD mice by up-regulating the expression of BDNF, Trk B, SYP and PSD95 in hippocampus.