Studies on Quality Control of Anti-inflammatory Extracts of Tetrastigma Hemsleyanum Diels Et Gilg's Aerial Part

OBJECTIVE To develop a method for studies on the quality control of anti-inflammatory extracts of Tetrastigma hemsleyanum Diels et Gilg's aerial part(THAA). METHODS Identify the THAA by TLC; the content of total flavonoids of THAA wene determined by colorimetric method; HPLC was established for the simultaneous determination of 7 pharmacodynamic components of THAA. Moreover, isoorientin was used as the internal reference in the determination of 7 pharmacodynamic components by quantitative analysis of multi-components by single-marker for THAA. RESULTS Six batches of THAA samples were chromatographed, and the fluorescent spots of the same color were displayed on the corresponding positions of the medicinal materials and the mixed reference materials on THAA. The components were well separated. The established colorimetric method showed a good linear relationship in the range of 9.63-57.79 μg×mL^-1. The regression equation was A=1.120×10^-2 C-2.400×10^-3, r=0.999 5. The average content of total flavonoids in 3 batches of THAA was 60.02%-60.32%. The content of chlorogenic acid, orientin, isoorientin, isoorientin-2'-O-rhamnoside, vitexin, isovitexin, vitexin-2''-O-rhamnoside in the anti-inflammatory extracts of THAA was about 21.64%, 8.04%, 6.61%, 19.51%, 2.03%, 1.59%, 4.03%; There was no significant difference between the content of 7 anti-inflammatory medicinal components in the THAA quantitative analysis of multi-components by single-marker multi-evaluation method and HPLC. CONCLUSION The established TLC identification, the determination method of total flavonoids, and the HPLC and quantitative analysis of multi-components by single-marker of the 7 pharmacodynamic components can be used as a reference for the internal quality control of the THAA.